A simple, rapid technique for determination of the effects of chemotherapeutic agents on mammalian cell-cycle traverse.

A simple, rapid protocol is described for separation of chemical agents into different classes, dependent upon their effects on cell-cycle traverse in line CHO Chinese hamster cells. The technique is a functional assay in that it measures the ability of synchronized cells to traverse the cell cycle on the basis of determination of the labeled and mitotic fractions in autoradiographs prepared from cultures receiving thymidine-3 H